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Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
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Microbiological Response to Periodontal Therapy: A Retrospective Study.

Vittorio Checchi1, Gaia Pascolo2

  • 1Department of Restorative Dentistry, University of Bologna, DIBINEM, Bologna, Italy.

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Summary

Osseous surgery significantly reduced periodontal pathogens and pocket depth in chronic periodontitis patients. This bacterial load reduction is key for long-term periodontal stability and treatment success.

Keywords:
Microbiological testMicrobiologyPeriodontal pathogensPeriodontal surgeryPeriodontal therapyRetrospective study

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Area of Science:

  • Periodontology
  • Microbiology
  • Oral Surgery

Background:

  • Periodontitis is a bacterial infection causing periodontal structure destruction.
  • Understanding pathogen dynamics is crucial for effective treatment.

Purpose of the Study:

  • To evaluate the presence and bacterial load of six key periodontal pathogens.
  • Assess changes before and after osseous surgery in chronic periodontitis patients.

Main Methods:

  • Cohort study of 38 severe chronic periodontitis patients.
  • Microbiological testing (Real-time PCR) for six pathogens pre- and post-surgery.
  • Analysis of bacterial load and clinical parameters (probing depth, bleeding, suppuration).

Main Results:

  • Osseous surgery led to significant reduction in probing depth, bleeding, and suppuration.
  • Total bacterial load and pathogen counts decreased significantly post-surgery.
  • Some pathogens (Aa, Pi) showed greater resistance; correlations found between clinical improvements and specific pathogen loads.

Conclusions:

  • Osseous surgery effectively reduces periodontal pathogens and pocket depth.
  • Lowering bacterial load is essential for achieving and maintaining periodontal stability.
  • This approach supports long-term successful periodontal treatment outcomes.