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[Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse
Jian Zhou1, Guang Ji, Jia-na Wen
1Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.
Objective:
To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.
Methods:
effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.
Results:
all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.
Conclusion:
ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.
