Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nucleolar dominance arises in Spartina homoploid hybrids and persists after allopolyploidization.

The Plant journal : for cell and molecular biology·2026

Same author

A label-free impedimetric immunosensor based on nitrogen-doped graphene acid for sensitive detection of vitamin D<sub>3</sub>.

Mikrochimica acta·2025

Same author

The profiling of phenolic compounds in archive Tokaj wines using liquid chromatography and antioxidant activity analysis.

Food chemistry: X·2025

Same author

Exogenously applied putrescine and chitosan-putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings.

Scientific reports·2025

Same author

What would a hypothetical supercyanobacterium look like?

Trends in biotechnology·2025

Same author

Characterization of Hemoglobin Variants by Capillary Electrophoresis, UV-Vis, and FTIR Spectroscopy.

Electrophoresis·2025

Search research articles

Related Experiment Video

Updated: Jun 22, 2026

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Published on: May 15, 2012

Automated nucleic acids isolation using paramagnetic microparticles coupled with electrochemical detection.

Dalibor Huska¹, Jaromir Hubalek, Vojtech Adam

¹Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic.

|June 30, 2009

Summary

This study presents an efficient method for isolating messenger RNA (mRNA) using paramagnetic microparticles and electrochemical detection. The optimized technique achieves high yields and can be automated for rapid analysis of biological samples.

More Related Videos

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Published on: June 11, 2013

Related Experiment Videos

Last Updated: Jun 22, 2026

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Published on: May 15, 2012

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

Published on: June 11, 2013

Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Accurate gene expression studies and chip technologies require efficient messenger RNA (mRNA) separation from biological samples.
Current methods for mRNA isolation can be complex and time-consuming.
Leveraging the polyadenine (poly(A)) tail of mRNA offers a target for selective binding and isolation.

Purpose of the Study:

To develop and optimize a method for easy, efficient, and low-demand separation and detection of mRNA.
To utilize paramagnetic microparticles coated with thymine chains for mRNA capture.
To couple this isolation method with sensitive electrochemical detection.

Main Methods:

Optimization of cyclic and square wave voltammetry for poly(A) detection, using poly(A) as an mRNA mimic.
Development of an automated approach for rinsing and hybridizing nucleic acids to paramagnetic microparticles.
Application of optimized conditions for mRNA isolation and subsequent electrochemical analysis.

Main Results:

Achieved a detection limit of 1 ng/ml for poly(A) under optimized square wave voltammetry (280 Hz, 200 s accumulation, acetate buffer pH 4.6, 35°C).
The automated isolation procedure yielded approximately 75% of poly(A).
Successfully applied the method to quantify mRNA in human brain tissues (40-760 µg mRNA/g tissue) and maize root extracts, with six samples processed in under 2.5 hours.

Conclusions:

The developed method provides an efficient, automatable, and sensitive approach for mRNA isolation and detection.
This technique is suitable for gene expression analysis and applications in chip technologies.
The method demonstrates versatility, being effective in analyzing clinical tissue samples and plant extracts.