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Related Concept Videos

Accuracy and Precision01:52

Accuracy and Precision

Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value.  Highly accurate measurements...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

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Related Experiment Video

Updated: Jun 22, 2026

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
11:22

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Published on: January 12, 2015

Accuracy and precision in quantitative fluorescence microscopy.

Jennifer C Waters1

  • 1Harvard Medical School, Department of Cell Biology, Boston, MA 02115, USA. jennifer_waters@hms.harvard.edu

The Journal of Cell Biology
|July 1, 2009
PubMed
Summary
This summary is machine-generated.

Quantitative fluorescence microscopy uses digital imaging to measure fluorescent molecules in cells. Understanding image acquisition parameters is crucial for accurate and precise results in biological research.

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Last Updated: Jun 22, 2026

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
11:22

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Published on: January 12, 2015

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
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Published on: September 26, 2014

Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

Published on: August 22, 2017

Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Light microscopy is essential for localizing fluorescent molecules in cell biology.
  • Advances in digital cameras and fluorophores have increased quantitative fluorescence microscopy.
  • Accurate measurements require understanding imaging system limitations.

Purpose of the Study:

  • To focus on digital image acquisition parameters affecting quantitative fluorescence microscopy.
  • To highlight the importance of proper system component usage for accurate measurements.

Main Methods:

  • Focus on digital image acquisition parameters.
  • Discuss limitations and proper use of imaging system components.

Main Results:

  • Digital image acquisition parameters significantly impact measurement accuracy and precision.
  • Proper understanding of the imaging system is vital for reliable quantitative data.

Conclusions:

  • Accurate quantitative fluorescence microscopy relies on careful control of image acquisition parameters.
  • Understanding and optimizing these parameters is key for reliable biological measurements.