In vitro protein synthesis by oligodendroglial cells

Oligodendroglial cells were isolated from spinal cord and brain stem tissue from 30-day-old rats according to the method of Poduslo and Norton except that acetylated trypsin at a concentration of 0.05% was used instead of unmodified trypsin, and the density gradient was slightly changed. Isolated oligodendroglial cells were found to incorporate [U-(14)C]leucine into TCA-insoluble protein up to 120 min. This incorporation was inhibited by cycloheximide and chloramphenicol. Purity of the cell preparation was assessed by phase contrast microscopy and by assay of 2',3'-cyclic nucleotide phosphohydrolase activity.