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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass Spectrometry: Complex Analysis

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Related Experiment Video

Updated: Jun 21, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

Phospholipid profiling by tandem mass spectrometry.

Zheng Cui1, Michael J Thomas

  • 1Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|July 28, 2009
PubMed
Summary

This review details analyzing glycerophospholipids using electrospray ionization with tandem mass spectrometry. This method bypasses chromatography for efficient lipid analysis from simple extracts.

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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
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A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Related Experiment Videos

Last Updated: Jun 21, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Lipidomics

Background:

  • Glycerophospholipids are crucial cellular components.
  • Accurate analysis of glycerophospholipids is vital for understanding biological processes.
  • Traditional lipid analysis often requires complex chromatographic separation.

Purpose of the Study:

  • To review methods for analyzing glycerophospholipids.
  • To highlight electrospray ionization with tandem mass spectrometry (ESI-MS/MS) as a primary analytical technique.
  • To discuss the advantages of direct analysis from lipid extracts.

Main Methods:

  • Electrospray ionization with tandem mass spectrometry (ESI-MS/MS).
  • Direct analysis of simple lipid extracts, avoiding chromatographic separation.
  • Mass spectrometry-based lipid identification and quantification.

Main Results:

  • ESI-MS/MS enables direct glycerophospholipid analysis.
  • The method allows for efficient characterization without prior separation.
  • Potential for high-throughput lipidomic studies.

Conclusions:

  • Direct ESI-MS/MS analysis is a powerful approach for glycerophospholipids.
  • This technique simplifies lipidomic workflows.
  • It offers a viable alternative to traditional chromatographic methods.