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Updated: Jun 21, 2026

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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Published on: June 11, 2016
Strategy for extracting DNA from clay soil and detecting a specific target sequence via selective enrichment and
Kweku K Yankson1, Todd R Steck
1Department of Biology, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Applied and Environmental Microbiology
|July 28, 2009
Abstract:
We present a simple strategy for isolating and accurately enumerating target DNA from high-clay-content soils: desorption with buffers, an optional magnetic capture hybridization step, and quantitation via real-time PCR. With the developed technique, microg quantities of DNA were extracted from mg samples of pure kaolinite and a field clay soil.
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