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Published on: May 24, 2020
[Recombinant goat pox virus expressing PPRV H protein]
Weiye Chen1, Linmao Qu, Sen Hu
1State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Summary
Researchers created a recombinant goat pox virus (GPV) vaccine expressing the Peste des petits ruminants virus (PPRV) H protein. This novel vaccine candidate successfully induced protective antibody responses against both GPV and PPRV in goats.
Area of Science:
- Veterinary Virology
- Molecular Biology
- Vaccinology
Context:
- Peste des petits ruminants (PPR) is a devastating viral disease affecting small ruminants.
- Goatpox virus (GPV) serves as a potential vector for developing novel vaccines.
- The H protein of PPRV is a key target for inducing protective immunity.
Purpose:
- To construct a recombinant GPV expressing the PPRV H protein (rGPV-PPRV-H).
- To verify the expression of the PPRV H protein in infected cells.
- To evaluate the immunogenicity and efficacy of the rGPV-PPRV-H vaccine candidate in goats.
Summary:
- Recombinant GPV expressing the PPRV H gene was successfully constructed and purified.
- Immunofluorescence and Western blotting confirmed the expression of PPRV H protein by the recombinant virus.
- Goats immunized with rGPV-PPRV-H developed significant neutralizing antibody titers against both GPV and PPRV.
Impact:
- This study provides a foundation for the development and industrialization of a PPRV recombinant GPV vaccine.
- The recombinant vaccine shows potential for controlling both goatpox and peste des petits ruminants.
- Successful development of this vaccine could significantly reduce economic losses in the sheep and goat industry.
