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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 21, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Isobaric peptide termini labeling for MS/MS-based quantitative proteomics.

Christian J Koehler1, Margarita Strozynski, Frank Kozielski

  • 1The Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway.

Journal of Proteome Research
|August 7, 2009
PubMed
Summary
This summary is machine-generated.

Isobaric peptide termini labeling (IPTL) enables precise proteome quantification. This novel method identifies and quantifies two differentially labeled states using MS/MS spectra for complex proteomic analyses.

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Last Updated: Jun 21, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Quantitative Biology

Background:

  • Isobaric peptide labeling is crucial for relative proteome quantification.
  • Existing methods require optimization for complex biological samples.

Purpose of the Study:

  • Introduce Isobaric Peptide Termini Labeling (IPTL) for dual-state proteome analysis.
  • Demonstrate IPTL's utility in identifying differentially regulated proteins.

Main Methods:

  • Peptide labeling at C-termini with MDHI/MDHI-d(4) and N-termini with SA/SA-d(4).
  • Utilizing MS/MS spectra for identification and quantification.
  • Employing Mascot score ratios for a dynamic quantification range.

Main Results:

  • Achieved a dynamic quantification range of 25 using Mascot score ratios.
  • Successfully identified differentially regulated apoptosis-linked proteins in HeLa cells treated with STLC.
  • Validated IPTL's suitability for complex proteome analysis.

Conclusions:

  • IPTL offers a novel and effective approach for relative proteome quantification.
  • The method is suitable for analyzing complex proteomes and identifying biological changes.
  • IPTL provides multiple quantification data points per peptide for enhanced analysis.