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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
Correlations between RNA and protein expression profiles in 23 human cell lines
Marcus Gry1, Rebecca Rimini, Sara Strömberg
1Department of Proteomics, School of Biotechnology, AlbaNova University Center, KTH Royal Institute of Technology, Stockholm, Sweden. marcus.gry@genpat.uu.se
BMC Genomics
|August 8, 2009
Summary
Researchers explored the link between RNA and protein levels across 1066 gene products in human cell lines. A third showed significant correlation, suggesting RNA profiles can indirectly support antibody specificity in immunoassays.
Area of Science:
- Molecular Biology
- Genomics
- Proteomics
Background:
- The Central Dogma (DNA -> RNA -> Protein) is a simplification; genome-wide RNA-protein level correlation is uncertain.
- Investigating the correlation between RNA and protein levels is crucial for understanding gene expression.
- Assessing this correlation helps validate biological models and experimental techniques.
Purpose of the Study:
- To quantify the genome-wide correlation between RNA and protein levels in human cell lines.
- To determine the extent to which RNA profiles can predict protein levels.
- To evaluate the utility of RNA profiling in supporting antibody specificity in immunoassays.
Main Methods:
- Compared RNA profiles (cDNA- and oligo-based microarrays) with protein profiles (immunohistochemistry).
- Analyzed 1066 gene products across 23 human cell lines.
- Utilized tissue microarrays for protein profiling.
Main Results:
- High mean correlation (0.52) between RNA levels from different platforms.
- Significant correlations ( > 0.445) between protein and RNA levels observed for one-third of gene products.
- Mean correlation coefficients between protein and RNA levels were 0.25 (cDNA) and 0.20 (oligo).
Conclusions:
- Significant correlations between RNA and protein levels were found for approximately one-third of the examined gene products.
- RNA profiling can offer indirect support for antibody specificity in immunoassays.
- A strong RNA-protein correlation suggests antibodies recognize their intended cognate antigens.
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