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Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
08:19

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Published on: December 19, 2011

A systems biology approach to understanding cis-regulatory module function.

Danuta M Jeziorska1, Kate W Jordan, Keith W Vance

  • 1Departments of Systems Biology and Biological Sciences, University of Warwick, Biomedical Research Institute, Gibbet Hill, Coventry CV4 7AL, UK.

Seminars in Cell & Developmental Biology
|August 8, 2009
PubMed
Summary

Scientists are cataloging cis-regulatory modules (CRMs), DNA elements controlling gene expression. Understanding CRM architecture and function is key to deciphering developmental biology and gene regulation networks.

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Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes
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Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes

Published on: May 31, 2011

Area of Science:

  • Genomics
  • Developmental Biology
  • Systems Biology

Background:

  • Cis-regulatory modules (CRMs) are functional DNA elements that encode genomic instructions.
  • CRMs precisely control the temporal and spatial patterns of gene expression during development.

Purpose of the Study:

  • To summarize recent advancements in cataloging and characterizing the complete repertoire of CRMs.
  • To define CRM architecture and their role as information processing devices within gene regulatory networks.

Main Methods:

  • Describing CRMs as clusters of transcription factor binding sites.
  • Positioning CRMs as nodes within large gene regulatory networks.
  • Overview of high-throughput techniques for genome-wide CRM identification and large-scale functional validation.

Main Results:

  • CRMs function as genomic information processing devices.
  • CRM architecture dictates how they process information for target genes.
  • A systems biology approach integrating computational and experimental methods is crucial.

Conclusions:

  • Cataloging and characterizing CRMs advances our understanding of gene regulation.
  • CRMs are fundamental to developmental processes.
  • Integrating diverse technologies enhances the study of CRM function.