Related Experiment Video
Updated: Jun 21, 2026
07:50
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
Published on: July 23, 2013
Heterelogous expression of plant genes
Filiz Yesilirmak1, Zehra Sayers
1Sabanci University, Orhanli, Tuzla, Istanbul, Turkey. filizy@sabanciuniv.edu <filizy@sabanciuniv.edu>
International Journal of Plant Genomics
|August 13, 2009
Summary
Heterologous expression enables producing plant proteins in simpler organisms for large-scale purification and functional studies. This review covers methods using bacteria, yeast, insect cells, and oocytes for protein characterization.
Area of Science:
- Biochemistry
- Molecular Biology
- Biotechnology
Background:
- Heterologous expression is crucial for producing plant proteins in alternative hosts.
- This facilitates large-scale purification for biochemical and biophysical analyses.
- Expression in model organisms aids in understanding protein functions within complex biological pathways.
Purpose of the Study:
- To provide an overview of recombinant plant protein production methods.
- To discuss the advantages of different expression systems for functional studies and protein characterization.
Main Methods:
- Review of established heterologous expression systems.
- Analysis of protein production in bacteria.
- Evaluation of protein production in yeast, insect cells, and Xenopus laevis oocytes.
Main Results:
- Each system (bacteria, yeast, insect cells, oocytes) offers distinct advantages for protein production.
- The choice of system impacts the efficiency of functional studies and protein characterization.
- Heterologous expression is a versatile tool for plant protein research.
Conclusions:
- Heterologous expression systems are vital for advancing plant protein research.
- Selecting the appropriate system is key for successful protein characterization and functional analysis.
- This technology supports diverse applications in biochemistry, biophysics, and systems biology.
Related Concept Videos
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
00:53Transgenic Organisms
Overview
01:59Plant Breeding and Biotechnology
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
02:11Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...
02:57Plant Tissue Culture
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.