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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Atomic Absorption Spectroscopy: Instrumentation01:22

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An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 20, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

Differential-absorption photoacoustic imaging.

Sheng-Wen Huang1, Janet F Eary, Congxian Jia

  • 1Department of Bioengineering, University of Washington, 1705 NE Pacific Street, Seattle, Washington 98195, USA. shengwen@u.washington.edu

Optics Letters
|August 18, 2009
PubMed
Summary
This summary is machine-generated.

Differential-absorption photoacoustic imaging enhances contrast by detecting absorption differences. This technique effectively suppresses unwanted objects, improving visualization of target molecules like PtOEP.

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Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces
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Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Spectroscopy

Background:

  • Photoacoustic imaging (PAI) offers non-ionizing, high-resolution cross-sectional imaging.
  • Contrast agents are crucial for PAI, but distinguishing between desired and undesired signals remains challenging.
  • Differential absorption techniques can improve specificity by exploiting spectral differences.

Purpose of the Study:

  • To introduce and validate differential-absorption photoacoustic imaging (DAPAI) for enhanced contrast.
  • To demonstrate the suppression of undesired objects using DAPAI.
  • To evaluate the effectiveness of DAPAI in complex imaging scenarios.

Main Methods:

  • Developed a DAPAI technique measuring the difference between transient and ground-state absorption.
  • Imaged two tubes: one with platinum(II) octaethylporphine (PtOEP) as the target, and another with IR-783 dye as the object to suppress.
  • Quantified signal suppression in decibels (dB).

Main Results:

  • Conventional PAI showed the IR-783 tube dominating the image.
  • DAPAI suppressed the IR-783 signal by 43 dB.
  • The PtOEP tube signal significantly overwhelmed the suppressed IR-783 signal in DAPAI.

Conclusions:

  • DAPAI effectively enhances contrast by suppressing undesired objects.
  • This technique improves the visualization of target molecules in complex backgrounds.
  • DAPAI shows promise for advanced molecular imaging applications.