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Updated: Jun 20, 2026

The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
Published on: November 2, 2021
NMR characterization for polysaccharide moiety of a glycopeptide
Libin Ye1, Jianrong Li, Jingsong Zhang
1Department of Biology, College of Food Science and Biotechnology, Zhejiang Gongshang University, Food Safety Key Lab of Zhejiang Province, Hangzhou 310035, China.
Abstract:
Ganoderma lucidum is a traditional medicinal herb used to treat various diseases in China and Southeast Asia for thousands of years. An aqueous glycopeptide, LZ-B-1, was prepared by successive chromatography and exhibited an immunostimulating potential. To better understand the mechanism of bioactivity for this compound, the polysaccharide moiety of glycopeptide LZ-B-1 was studied by NMR spectroscopy. The results indicated that the polysaccharide moiety had a backbone of 1,6-disubstituted-alpha-galactopyranosyl, 1,2,6-trisubstituted-alpha-galactopyranosyl, 1,3-disubstituted-beta-glucopyranosyl and 1,4,6-trisubstituted-beta-glucopyranosyl residues. The branches were mainly composed of 1-substituted-beta-glucopyranosyl and 1-substituted-alpha-fucopyranosyl residues.
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