Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Coupling kinetics of the photorefractive hologram.

Applied optics·2010

Same author

Performance of a phase-transformed input joint transform correlator.

Applied optics·2010

Same author

Kinoform-based Nipkow disk for a confocal microscope.

Applied optics·2010

Same author

Temperature-compensated fiber specklegram strain sensing with an adaptive joint transform correlator.

Applied optics·2010

Same author

Multilayer associative memory and its hybrid optical implementation.

Applied optics·2010

Same author

Diffraction properties of a reflection photorefractive hologram: authors' reply to comments.

Applied optics·2010

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Published on: January 14, 2020

High-resolution rainbow holographic process.

F T Yu, P H Ruterbusch, S L Zhuang

|August 21, 2009

Summary

This summary is machine-generated.

This study introduces a high-resolution, one-step rainbow holography technique using a cylindrical lens. The new method enhances image resolution and reduces noise and color blur compared to conventional approaches.

More Related Videos

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Related Experiment Videos

Last Updated: Jun 20, 2026

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Recording Ultra-Realistic Full-Color Analog Holograms for Use in a Moving Hologram Display

Published on: January 14, 2020

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

Optics
Holography
Image Processing

Background:

Conventional one-step rainbow holography techniques can suffer from limitations in resolution and image quality.
Reducing speckle noise and color blur is crucial for practical holographic applications.

Purpose of the Study:

To present a novel high-resolution one-step rainbow holography technique.
To demonstrate the effectiveness of using a cylindrical lens in this holographic process.
To compare the performance against conventional methods.

Main Methods:

Development of a one-step rainbow holography setup incorporating a cylindrical lens.
Experimental validation under diverse holographic conditions.
Comparative analysis of image resolution, speckle noise, and color blur.

Main Results:

The cylindrical lens technique achieved higher hologram-image resolution.
A significant reduction in speckle noise was observed.
The color-blur effect was notably decreased compared to the conventional method.
The technique proved particularly effective for 2-D object transparencies.

Conclusions:

The proposed cylindrical lens-based one-step rainbow holography offers superior image quality.
This method presents a viable advancement for applications requiring high-resolution holographic imaging.
It is especially well-suited for imaging 2-D objects.