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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Updated: Jun 20, 2026

An Unbiased Approach of Sampling TEM Sections in Neuroscience
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Published on: April 13, 2019

Moiré topography, sampling theory, and charged-coupled devices.

B W Bell, C L Koliopoulos

    Optics Letters
    |September 2, 2009
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel moiré contouring technique using undersampled cosine fringes and a charged-coupled-device detector. This method eliminates unwanted terms and spurious fringes, enhancing contour fringe visibility and sensitivity.

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    Area of Science:

    • Optics and Photonics
    • Metrology
    • Image Processing

    Background:

    • Classical moiré contouring methods suffer from unwanted sum, shadow, and grid terms.
    • Spurious moiré fringes often arise from higher harmonics in traditional techniques.
    • A reference grid is typically required for conventional projection-type moiré contouring.

    Purpose of the Study:

    • To develop a reference-grid-free projection-type moiré contouring technique.
    • To eliminate unwanted terms and spurious fringes in moiré contouring.
    • To enhance the visibility and sensitivity of moiré contour fringes.

    Main Methods:

    • Undersampling projected cosine fringes using a charged-coupled-device (CCD) detector array.
    • Eliminating sum, shadow, and grid terms by exploiting aliasing effects.
    • Applying higher-order aliasing for increased measurement sensitivity.

    Main Results:

    • A high-visibility sampled version of moiré difference contour fringes is produced.
    • Elimination of unwanted classical moiré terms and spurious higher-harmonic fringes.
    • Demonstration of increased sensitivity through higher-order aliasing.

    Conclusions:

    • The proposed technique offers a simplified and more robust approach to moiré contouring.
    • The method effectively overcomes limitations of classical moiré techniques.
    • The findings extend the Whittaker-Shannon sampling theorem to moiré applications.