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Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model
Published on: August 22, 2015
Particle size analysis of nanocrystals: improved analysis method.
1Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia. ck@ckc-berlin.de
International Journal of Pharmaceutics
|September 8, 2009
Summary
An optimized analysis method for nanocrystal size is crucial for accurate physical stability assessment. Saturating the dispersion medium prevents dissolution, ensuring reproducible and reliable results in nanosuspension development.
Area of Science:
- Pharmaceutical technology
- Materials science
- Analytical chemistry
Background:
- Accurate particle size determination is critical for nanosuspension stability.
- Conventional laser diffraction methods may yield misleading results due to nanocrystal dissolution.
- Optimized analysis techniques are needed to overcome limitations in current methods.
Purpose of the Study:
- To compare conventional and optimized laser diffraction analysis for nanosuspensions.
- To investigate the influence of optical parameters and measurement techniques.
- To assess and mitigate nanocrystal dissolution during particle size analysis.
Main Methods:
- Comparison of laser diffraction data from conventional and optimized analysis methods.
- Evaluation of nanocrystal dissolution in unsaturated and saturated media.
- Analysis of 40 different nanosuspensions using both methods.
Main Results:
- Nanocrystal dissolution occurs in unsaturated media, leading to erratic and irreproducible results.
- Saturating the dispersion medium with nanocrystals prevents dissolution and ensures reliable measurements.
- The optimized method revealed significant instability in 36 out of 40 nanosuspensions, contrary to conventional method findings.
Conclusions:
- Optimized analysis is essential for reliable physical stability assessment of nanosuspensions.
- Early identification of unstable formulations saves time and resources in drug development.
- The optimized method reliably discriminates between stable and unstable nanosuspensions at early stages.
