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Updated: Jun 20, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
Published on: May 1, 2013
Suppressed-priming PCR, a novel concept of DNA quantification based on PCR kinetics
Shuhei Takemoto1, Hiroaki Okada
1National Institute for Agro-Environmental Sciences, 3-1-3 Kannon-dai, Tsukuba, Ibaraki 305-8604, Japan.
Abstract:
The concept of an unbiased DNA quantification method is proposed based on PCR kinetics. A set of PCRs was performed with a dilution series of primers, which differently limited DNA amplification. The difference in amplification efficiency between the DNA templates reflected the estimates of their relative amounts. This concept might serve as a theoretical base for further development of accurate and robust PCR-based quantification methods.
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