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Published on: August 13, 2013
[Differentiation of recombinant adenoviruses expressed MDV-1 VP22 subcellular localization pattern]
Chen-fei Zhang1, Ai-jian Qin, Juan-juan Chen
1Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. zhangchenfei1981@163.com
Aim:
To study the transduction and localization mechanism of Marek's disease virus serotype 1 (MDV-1) CVI988 VP22 in different cells.
Methods:
VP22 was expressed with recombinant adenovirus and identified by immunofluorescence assay (IFA) and Western blot. Lysates of recombinant virus infected 293 cells were added to normal MDBK cells to identify the transduction property of VP22. AD-293 cells infected with recombinant virus were fixed to investigate localization of VP22 at different time post infection. Transient expression of VP22 in AD-293 cells was carried out for control.
Results:
The results showed that the VP22 expressed by recombinant adenovirus entered almost all the monolayer cells, which indicate the VP22 remains its transduction property. The VP22 first gather round the nucleus membrane, and then concentrated in particles in cytoplasm of 293 cells infected with recombinant adenovirus, compared with the nuclei localization pattern of VP22 in MDV infected CEF and transient expressed VP22 in 293 cells.
Conclusion:
The VP22 presented a different localization pattern in cells infected with different recombinant virus.
