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Published on: September 9, 2011
Interferon-gamma bolsters CD95/Fas-mediated apoptosis of astroglioma cells
Anna M Giammarioli1, Rosa Vona, Lucrezia Gambardella
1Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanita', Rome, Italy. anna.giammarioli@iss.it
Abstract:
In the present study, we investigated the mechanisms of the resistance to CD95-mediated cell death and the effects of interferon-gamma in modulating the susceptibility to CD95-induced apoptosis of human astroglioma cells. We found that interferon-gamma administration sensitized cancer cells to CD95-mediated apoptosis. The mechanism underlying this sensitization appeared to be associated with a framework of cell changes, including up-regulation of death receptor at the cell surface, pro-apoptotic molecule Bax and Bak over-expression and mitochondria hyperpolarization, as is known to be associated with cell sensitization to apoptosis. An involvement of the proteasome activity in the mechanism of sensitization by interferon-gamma was also detected, probably as a result of the differing expression of catalytic proteasome subunits. Taken together, these findings suggest that interferon-gamma could represent a promising candidate for modulating astroglioma cell apoptotic susceptibility.
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