Synthesis and properties of terminally modified oligonucleotides capable of short-RNA selective hybridization
Kohji Seio1, Kazuya Miyazaki, Sayako Kurohagi
1Department of Life Science, Graduate School of Bioscience and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kseio@bio.titech.ac.jp
Abstract:
We have developed 2'-O-methyl-RNAs having phosphorylated cyclohexane at the 5' and/or 3'-terminal adenine. These 2'-O-methyl-RNAs formed less stable duplex with the longer target RNA than that with the short target RNA. We tried to improve the short-RNA selective hybridization property by the synthesis of 2'-O-methyl-RNAs having terminal guanine modifications.
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