Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Protein Modifications in the RER01:26

Protein Modifications in the RER

Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive identification and functional analysis of fully disordered proteins essential for cell survival.

RNA (New York, N.Y.)·2025
Same author

Abundant piRNA production mediated by the <i>Drosophila</i> GTSF1 homolog Tpp ensures Aubergine localization and germ plasm assembly.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Autonomous shaping of the piRNA sequence repertoire by competition between adjacent ping-pong amplification sites.

Molecular cell·2025
Same author

No structure, no problem: Protein stabilization by Hero proteins and other chaperone-like IDPs.

Biochimica et biophysica acta. General subjects·2025
Same author

Transposon-host arms race: a saga of genome evolution.

Trends in genetics : TIG·2025
Same author

Swift induction of human spinal lower motor neurons and robust ALS cell screening via single-cell imaging.

Stem cell reports·2024
Same journal

A novel convenient method for high bacteriophage titer assay.

Nucleic acids symposium series (2004)·2009
Same journal

Expression behavior of high-pressure-compacted plasmid DNA in mammalian cell.

Nucleic acids symposium series (2004)·2009
Same journal

Role of exposed aromatic residues in substrate-binding of CBM family 5 chitin-binding domain of alkaline chitinase.

Nucleic acids symposium series (2004)·2009
Same journal

Incipient complex formation between AP endonucleases and DNA containing AP site: a vital role of the tryptophan residue.

Nucleic acids symposium series (2004)·2009
Same journal

Physiological role of RsgA in ribosome biosynthesis.

Nucleic acids symposium series (2004)·2009
Same journal

Trans-translation by tmRNA and SmpB.

Nucleic acids symposium series (2004)·2009
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Cerebellar Regional Dissection for Molecular Analysis
08:51

Cerebellar Regional Dissection for Molecular Analysis

Published on: December 5, 2020

Biochemical dissection of RISC assembly and function.

Yukihide Tomari1

  • 1Department of Medical Genome Sciences, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan. tomari@iam.u-tokyo.ac.jp

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed
Summary
This summary is machine-generated.

Small silencing RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs), regulate gene expression through the RNA-induced silencing complex (RISC). This study reveals distinct assembly and function pathways for siRNA- and miRNA-loaded RISC in Drosophila.

More Related Videos

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
05:45

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Published on: May 3, 2024

Related Experiment Videos

Last Updated: Jun 20, 2026

Cerebellar Regional Dissection for Molecular Analysis
08:51

Cerebellar Regional Dissection for Molecular Analysis

Published on: December 5, 2020

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples
05:45

Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples

Published on: May 3, 2024

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Small silencing RNAs, such as siRNAs and miRNAs, are key regulators of gene expression.
  • These RNAs function via the RNA-induced silencing complex (RISC).
  • RISC assembly and function are complex and diverse, involving target cleavage, translational repression, and deadenylation.

Purpose of the Study:

  • To investigate the distinct pathways of RISC assembly for siRNAs and miRNAs.
  • To elucidate the differential functions of siRNA- and miRNA-loaded RISC.
  • To utilize Drosophila melanogaster as a model organism for studying these processes.

Main Methods:

  • Utilized Drosophila melanogaster as a model system.
  • Investigated the assembly pathways of RISC loaded with siRNAs.
  • Examined the assembly pathways of RISC loaded with miRNAs.
  • Analyzed the functional differences between siRNA- and miRNA-mediated silencing.

Main Results:

  • Demonstrated that siRNAs and miRNAs are assembled into distinct types of RISC.
  • Showed that these distinct RISC types exhibit different functional mechanisms.
  • Provided insights into the ordered pathways governing RISC assembly in Drosophila.

Conclusions:

  • siRNAs and miRNAs are processed and loaded into RISC through separate pathways.
  • The distinct RISC complexes mediate gene silencing via different mechanisms.
  • Drosophila serves as a valuable model for dissecting the intricacies of RNA silencing pathways.