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Updated: Feb 1, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Synthesis and triplex formation of oligonucleotides containing 8-thioxodeoxyadenosine and
Akihiro Ohkubo1, Kenichi Miyata, Hirosuke Tsunoda
1Department of Life Science, Graduate School of Bioscience and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Abstract:
For more effective DNA triplex formation under neutral conditions, we synthesized triplex-forming oligonucleotides (TFO) containing 8-thioxodeoxyadenine (s(8)A) residues in place of the protonated cytosines (Cs) required for the third base pairing with DNA duplexes. Consequently, it was found that s(8)A exhibited much stronger hybridization ability than C under neutral conditions when four s(8)A bases were arranged in a consecutive sequence. Moreover, we also synthesized TFOs containing 5-methyl-2-thiocytosines and examined their ability of triplex formation.
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