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Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

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Updated: Jun 20, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Polyethylenimine derived nanoparticles for efficient gene delivery.

A Pathak1, S Patnaik, K C Gupta

  • 1Institute of Genomics and Integrative Biology, Delhi University Campus, Delhi 110007, India.

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed
Summary
This summary is machine-generated.

New nanoparticles made from branched polyethylenimine (bPEI) and polysaccharides show improved safety and gene delivery efficiency for non-viral gene therapy. These bPEI nanoparticles offer a promising alternative to standard transfection reagents.

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Assembly and Characterization of Polyelectrolyte Complex Micelles
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Assembly and Characterization of Polyelectrolyte Complex Micelles

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Assembly and Characterization of Polyelectrolyte Complex Micelles
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Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Area of Science:

  • Biotechnology
  • Gene Therapy
  • Nanomedicine

Background:

  • Non-viral gene delivery faces challenges in safety and efficiency.
  • Branched polyethylenimine (bPEI) is a common non-viral vector.
  • Developing effective and safe gene therapy vectors is crucial.

Purpose of the Study:

  • To develop novel bPEI-based nanoparticles for enhanced gene therapy.
  • To evaluate the physicochemical properties, cytotoxicity, and transfection efficiency of these nanoparticles.
  • To assess the in vivo targeting efficacy of the optimized nanoparticles.

Main Methods:

  • Synthesized bPEI (25 kDa) nanoparticles using electrostatic interactions with alginic acid (Al) and hyaluronic acid (HA).
  • Varied polysaccharide amounts to create a library of PEI-Al and PEI-HA nanoparticles.
  • Characterized nanoparticles for size, surface charge, cytotoxicity, and transfection efficiency in vitro.
  • Evaluated in vivo targeting in tumor-induced mice using PEI-HA nanoparticles.

Main Results:

  • PEI-Al and PEI-HA nanoparticles demonstrated superior cell viability compared to native bPEI.
  • Nanoparticles exhibited significantly higher transfection efficiency than native bPEI and standard reagents.
  • PEI-HA(4.6%) nanoparticles showed promising in vivo targeting efficacy in a tumor model.

Conclusions:

  • bPEI-polysaccharide nanoparticles represent a safe and efficient non-viral gene delivery system.
  • These nanoparticles overcome limitations of native bPEI and conventional transfection agents.
  • Further investigation of PEI-HA nanoparticles holds potential for advanced gene therapy applications.