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Updated: Jan 9, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
Published on: May 2, 2025
Promotion of strand invasion by utilizing entropically-favored PNA
Takumi Ishizuka1, Stefano Sforza, Makoto Komiyama
1Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.
Abstract:
Invasion of peptide nucleic acid (PNA) is important to target a specific site in double-stranded DNA. However, it needs improvement in many ways such as sequence versatility and invasion efficiency. It is here reported that one 10-mer strand of alpha-lysine substituted peptide nucleic acid (PNA) efficiently invades double-stranded DNA (dsDNA) in the presence of single-strand binding protein (SSB) via Watson-Crick rule. According to the thermodynamic parameters in this study, highly efficient invasion was achieved by a favorable entropy change. The importance of preorganization of PNAs for efficient strand invasion is indicated.
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