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Photo-switching of vinylpyrene-substituted 2'-deoxyguanosine and its application

Yoshio Saito1, Yoshiki Takeuchi, Katsuhiko Matsumoto

  • 1Department of Materials Chemistry and Engineering and NEWCAT Institute, School of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saito@chem.ce.nihon-u.ac.jp

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|September 15, 2009
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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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