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Published on: September 7, 2017
Osmium complex binding to mismatched methylcytosine: effect of adjacent bases
Akiko Nomura1, Kazuki Tainaka, Akimitsu Okamoto
1RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Abstract:
We investigated the efficiency of osmium complex formation at 5-methylcytosine in mismatched DNA duplexes. Osmium complexation was not observed in fully matched duplexes, whereas the complexation site and efficiency in mismatched duplexes depended on the 5'-neighboring base of the 5-methylcytosine. In particular, when the base adjacent to the 5' side of the mismatched base pair was thymine, a unique side reaction was observed. However, the mismatched base pairs did not influence the selectivity of osmium complexation with methylated DNA.
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