Inhibition of influenza virus by baculovirus-mediated shRNA
Hitoshi Suzuki1, Hiroshi Saitoh, Tomoyuki Suzuki
1Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology and High Technology Research Center, Chiba Institute of Technology, Narashino Chiba 275-0016, Japan.
Abstract:
Influenza viruses A and B cause widespread infections of the human respiratory tract; however, existing vaccines and drug therapy are of limited value for their treatment. Here we show that bispecific short hairpin small-interfering RNA constructs containing an eight-nucleotide intervening spacer, targeted against influenza virus A or influenza virus B, can inhibit the production of both types of virus in infected cell lines. This multiple vector showed remarkable ability to cope with both influenza virus A or B. Furthermore, the Autographa californica multiple nuclear polyhedrosis virus can infect a range of mammalian cells, facilitating its use as a baculovirus vector for gene delivery into cells. In this study, baculovirus-mediated bispecific short-hairpin RNA expression markedly inhibited both influenza virus A and B production.
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