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Related Concept Videos

Tissue Membranes01:27

Tissue Membranes

A tissue membrane is a thin layer of cells that covers the outside of the body, the organs, internal passageways that lead to the exterior of the body, and the lining of the moveable joint cavities. There are two basic types of tissue membranes— connective tissue and epithelial membranes.
Connective Tissue Membranes
The connective tissue membrane is formed solely from connective tissue. These membranes encapsulate organs, such as the kidneys, and line our movable joints. A synovial membrane is...

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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
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Porous hollow membrane sheet for tissue engineering applications.

Afra Hadjizadeh1, Davod Mohebbi-Kalhori

  • 1Department of Chemical Engineering and Biotechnology, University of Sherbrooke, 2500 University Boulevard, Sherbrooke, Québec, Canada J1K 2R1. afra.hajizadeh@gmail.com

Journal of Biomedical Materials Research. Part A
|September 22, 2009
PubMed
Summary

Researchers developed a novel porous hollow membrane sheet (PHMsh) to act as both a scaffold and capillary bed for tissue engineering. This innovation supports nutrient supply, crucial for growing thick tissue constructs in vitro.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Creating thick, functional tissue constructs in vitro remains a significant challenge in tissue engineering.
  • Effective nutrient and waste transport, mimicking a capillary bed, is essential for engineered tissue survival and growth.
  • Existing methods for fabricating capillary-like architectures are complex and difficult to implement.

Purpose of the Study:

  • To introduce a simple method for fabricating a porous hollow membrane sheet (PHMsh).
  • To design the PHMsh to function as both a scaffold and an integrated capillary bed.
  • To evaluate the PHMsh's potential for supporting cell adhesion and enabling large tissue mass generation.

Main Methods:

  • Fabrication of the PHMsh using poly(epsilon-caprolactone) via solvent casting (immersion precipitation and air casting).
  • Morphological analysis using optical and scanning electron microscopy.
  • Surface modification with n-heptylamine plasma polymer (HApp) and characterization via X-ray photoelectron spectroscopy.
  • In vitro cell adhesion studies using human umbilical vein endothelial cells (HUVECs) and fibroblasts.

Main Results:

  • Successful fabrication of a flexible, porous hollow membrane sheet with parallel channels.
  • Confirmation of successful HApp surface coating, enhancing cell adhesion.
  • Demonstrated enhanced adhesion of both HUVECs and fibroblasts on the PHMsh.
  • Preliminary results indicate the PHMsh's suitability as a scaffold for tissue engineering.

Conclusions:

  • The developed PHMsh offers a promising, simple solution for creating integrated capillary beds within tissue engineering scaffolds.
  • The PHMsh can be utilized in various configurations (flat, rolled, sandwiched) to support tissue growth.
  • This technology holds potential for generating large tissue masses in vitro, particularly within perfusion systems.