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Updated: Jun 20, 2026
Model of Ischemia and Reperfusion Injury in Rabbits
Published on: November 3, 2023
[Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus]
Zheng Ni1, Wei Wei, Guang-Qing Liu
1Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Abstract:
The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.
Insights
Rabbit hemorrhagic disease virus (RHDV) infection induces apoptosis in RK13 cells, characterized by DNA fragmentation and Caspase 3 activation, suggesting apoptosis is key to RHD pathogenesis.
Area of Science:
- Cell Biology
- Virology
- Pathogenesis
Context:
- Rabbit hemorrhagic disease (RHD) is a severe and often fatal disease in rabbits.
- Rabbit hemorrhagic disease virus (RHDV) is the causative agent of RHD.
- Understanding the mechanisms of RHDV-induced cell death is crucial for disease management.
Purpose:
- To investigate the induction of apoptosis in RK13 cells by RHDV infection.
- To elucidate the cellular and molecular events associated with RHDV-induced apoptosis.
Summary:
- RHDV infection triggers apoptosis in RK13 cells, evidenced by DAPI staining revealing nuclear morphological changes.
- DNA fragmentation, observed as a DNA ladder at 24, 48, and 72 hours post-infection, confirms RHDV-induced DNA damage.
- Flow cytometry indicated approximately 63% of cells undergoing apoptosis by 48 hours post-infection, alongside demonstrated Caspase 3 activation.
Impact:
- This study demonstrates that apoptosis plays a significant role in the pathogenesis of RHD.
- Findings contribute to understanding RHDV's cellular mechanisms and potential therapeutic targets.
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