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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Updated: Jun 20, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Genetically encoded biosensors based on engineered fluorescent proteins.

Wolf B Frommer1, Michael W Davidson, Robert E Campbell

  • 1Carnegie Institution for Science, 260 Panama St., Stanford, CA 94305, USA. wfrommer@stanford.edu

Chemical Society Reviews
|September 23, 2009
PubMed
Summary
This summary is machine-generated.

Fluorescent proteins enable non-invasive cell biology research. Recent advances focus on developing these proteins into biosensors for imaging cellular components and activities in living cells.

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

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Last Updated: Jun 20, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Imaging

Background:

  • Fluorescent proteins (FPs) are crucial tools in cell biology.
  • Common uses include imaging protein expression, localization, and dynamics.
  • There's a rising interest in FPs for biosensing applications.

Purpose of the Study:

  • To provide an overview of fluorescent protein-based biosensor development.
  • To highlight progress in creating biosensors for cellular analysis.

Main Methods:

  • Review of existing literature on fluorescent protein biosensors.
  • Analysis of applications in imaging ions, molecules, enzyme activity, and protein conformation.

Main Results:

  • Fluorescent proteins are increasingly used as biosensors.
  • Biosensors allow minimally invasive imaging of various cellular parameters.
  • Significant progress has been made in developing FP-based biosensors.

Conclusions:

  • Fluorescent protein-based biosensors represent a significant advancement in cell biology.
  • These biosensors offer powerful tools for real-time, in vivo cellular analysis.
  • The field continues to evolve with new sensor designs and applications.