[Relation between human platelet aggregation and activation of soluble platelet guanylate cyclase]

Guanylate cyclase from thrombocytes with elevated ability to aggregation (diabetes mellitus) exhibited a decreased rate of activity and low response to stimulation by sodium nitroprusside and protoporphirin IX. The phenomenon observed did not depend on hem-deficiency of guanylate cyclase and was manifested most distinctly in the II type of diabetes mellitus as compared with the I type of the disease. Experimental data as well as the previously obtained results about more elevated rate of thrombocytes aggregation in patients with the II type of diabetes mellitus demonstrated that regulating functions of the cGMP system in the cells aggregation were impaired and that aggregation of thrombocytes appears to depend on the guanylate cyclase activity.