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A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

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Published on: December 23, 2016

Functional disulfide-stabilized polymer-protein particles.

Zhongfan Jia¹, Jingquan Liu, Cyrille Boyer

¹Centre for Advanced Macromolecular Design (CAMD), School of Chemical Sciences and Engineering, The University of New South Wales (UNSW), Sydney, NSW 2052, Australia.

Biomacromolecules

|October 1, 2009

Summary

This summary is machine-generated.

We developed versatile polymer-protein hybrid particles (PPHPs) for drug delivery and imaging. These disulfide cross-linked, poly(ethylene glycol)-streptavidin particles offer tunable sizes and functional surfaces for targeted applications.

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Area of Science:

Biomaterials Science
Nanotechnology
Polymer Chemistry

Background:

Polymer-protein hybrid particles (PPHPs) show promise in drug delivery, diagnostics, and biomedical imaging.
Existing PPHPs often require complex synthesis or lack tunable properties for specific applications.

Purpose of the Study:

To develop a straightforward method for creating disulfide cross-linked, poly(ethylene glycol)-streptavidin hybrid particles with controllable dimensions.
To engineer PPHPs with accessible biotin-binding sites for versatile bioconjugation and functional core for drug/label attachment.

Main Methods:

Synthesis of alpha-biotin PEG-b-poly(pyridyldisulfide ethylmethacrylate) block copolymers via RAFT polymerization.
One-pot micellization, disulfide cross-linking, and core functionalization using a thiol-reactive maleimide fluorophore.
Characterization of particle morphology, size, biotin availability (streptavidin/HABA assay), and streptavidin decoration via TEM and confocal microscopy.

Main Results:

Spherical micelles with a diameter of 54 ± 4 nm were formed, featuring a biotinylated poly(ethylene glycol) corona and a disulfide cross-linked core.
High biotin availability (75 mol %) on the micelle surface was confirmed, enabling efficient streptavidin conjugation.
Streptavidin-decorated PPHPs formed aggregates with tunable sizes ranging from 350 nm to 2 µm, with streptavidin localized on the particle periphery.

Conclusions:

A facile method for producing tunable, disulfide cross-linked polymer-protein hybrid particles with accessible streptavidin-binding sites was established.
These PPHPs offer significant versatility for applications in drug delivery, diagnostics, and imaging due to their functionalizable surface and core.
The tunable dimensions and controlled surface functionalization of these hybrid particles make them promising candidates for advanced biomedical applications.