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How to measure forces with atomic force microscopy without significant influence from nonlinear optical lever
Esben Thormann1, Torbjön Pettersson, Per M Claesson
1Department of Chemistry, Surface and Corrosion Science, Royal Institute of Technology, Drottning Kristinas Vag 51, SE-100 44 Stockholm, Sweden. esben.thormann@surfchem.kth.se
The Review of Scientific Instruments
|October 2, 2009
Summary
The optical lever technique in atomic force microscopy (AFM) shows a nonlinear detector response, impacting studies using soft or short cantilevers. This nonlinearity, often overlooked, necessitates careful cantilever selection for accurate AFM measurements.
Area of Science:
- Physics
- Materials Science
- Nanotechnology
Background:
- Atomic Force Microscopy (AFM) commonly uses an optical lever technique to measure cantilever deflection.
- A nonlinear relationship between the detected signal and actual cantilever deflection is often ignored in AFM literature.
- This nonlinearity is typically assumed to only affect measurements with large cantilever deflections.
Purpose of the Study:
- To investigate the reasons behind the nonlinear detector response in AFM's optical lever technique.
- To compare experimental findings with theoretical calculations regarding this nonlinearity.
- To determine the impact of this nonlinearity on AFM studies, particularly those using soft or short cantilevers.
Main Methods:
- Designed specific experiments to probe the nonlinear detector response.
- Performed calculations to correlate with experimental observations.
- Analyzed the influence of cantilever properties on the nonlinearity.
Main Results:
- Experimental results confirm a significant nonlinear relationship between the detected signal and cantilever deflection.
- The nonlinearity is shown to affect AFM studies even with soft or short cantilevers, contrary to common assumptions.
- Identified the primary cause of the nonlinearity through analysis.
Conclusions:
- The nonlinear detector response in AFM's optical lever technique is a critical factor affecting measurement accuracy.
- Soft or short cantilevers are particularly susceptible to this nonlinearity, impacting data interpretation.
- A practical guideline for selecting appropriate cantilevers under various experimental conditions is proposed based on the findings.

