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Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Overview of Cell Signaling01:23

Overview of Cell Signaling

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Overview of Cell Signaling01:23

Overview of Cell Signaling

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate with the environment.
Cells respond to many types of information, often through receptor proteins positioned on the membrane. For example, skin cells respond to and transmit touch...
Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...

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Related Experiment Video

Updated: Jun 19, 2026

Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles
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Published on: November 3, 2023

Current Screens Based on the AlphaScreen Technology for Deciphering Cell Signalling Pathways.

Saïd Taouji1, Sophie Dahan, Roger Bossé

  • 1Avenir, INSERM U889, Montreal, Qc, Canada.

Current Genomics
|October 2, 2009
PubMed
Summary
This summary is machine-generated.

The Alphascreen technology offers a sensitive, bead-based method for studying cell signaling pathways. This review details its broad applications in understanding molecular interactions and enzymatic activities in signal transduction.

Keywords:
Alphascreen™.Signal transductionsignaling

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Signaling

Background:

  • Post-genomic research faces challenges in fully characterizing signal transduction pathways.
  • Understanding the roles and interactions of signaling intermediates requires integrated approaches.
  • Sensitive, high-throughput technologies are essential for global molecular studies of cell signaling.

Purpose of the Study:

  • To review the literature on Alphascreen technology applications in signal transduction research.
  • To highlight the utility of Alphascreen for studying enzymatic activities and biomolecular interactions.
  • To showcase the broad spectrum of Alphascreen-based methods for analyzing cell signaling pathways.

Main Methods:

  • Literature review of Alphascreen technology applications.
  • Focus on bead-based, homogenous assay formats.
  • Analysis of Alphascreen's role in detecting signaling events.

Main Results:

  • Alphascreen is a valuable tool for quantifying signaling events.
  • The technology supports the study of enzymatic activities.
  • It enables the detection of diverse biomolecular interactions within signaling networks.
  • Demonstrates broad applicability in signal transduction pathway analysis.

Conclusions:

  • Alphascreen technology provides a robust platform for signal transduction research.
  • Its homogenous, bead-based approach facilitates high-throughput analysis.
  • This technology is crucial for advancing the integrated study of cell signaling pathways.