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Updated: Jun 19, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
Vascular Endothelial Growth Factor Production is Regulated by Gene Polymorphisms
Mojgan Mohammadi1, Mohammad Reza Bazrafshani, Philip J Day
1Department of Immunology, Kerman University of Medical Sciences, Iran. mo_mohammadi@kmu.ac.ir
Background:
Vascular endothelial growth factor (VEGF) has a key role in angiogenesis and in transplantation. The level of VEGF is related to the differences in the DNA sequence of its promoter region.
Objectives:
In this study, the association between the combination of VEGF -1154 G and -2578 C alleles and VEGF production by LPS-stimulated PBMCs was investigated. In addition; the relationship between VEGF polymorphisms and the influence of TNF- and IL-4 on VEGF production was studied.
Methods:
VEGF -1154 G/A and -2578 C/A were detected using ARMS-PCR. To determine the impact of combinations of these two polymorphisms on VEGF production; PBMCs were stimulated by LPS and VEGF production was measured by ELISA.
Results:
The combinations of -1154 GG/-2578 CC and -1154 GG/-2578 CA were significantly associated with higher VEGF production (p<0.0001). Production of VEGF was significantly influenced by TNF- in individuals who had certain VEGF genotype combinations. Although VEGF production was dramatically suppressed by IL-4, it was not dependent on VEGF genotype.
Conclusions:
Since TNF- has influence on the graft outcome, to avoid allocation of grafts from high TNF- producer donors to recipients, it might be useful to predict and minimize graft rejection by having prior knowledge of TNF- and also VEGF genotypes especially -1154 G/A and -2578 C/A VEGF.
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