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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
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Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
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Spatially coherent white-light interferometer based on a point fluorescent source.

H H Liu, P H Cheng, J Wang

    Optics Letters
    |October 6, 2009
    PubMed
    Summary
    This summary is machine-generated.

    We created a novel white-light interferometer using a point fluorescent source for advanced imaging and measurements. This system enables high-resolution reflectometry and precise group-velocity-dispersion analysis.

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    Area of Science:

    • Optics and Photonics
    • Interferometry
    • Fluorescence Spectroscopy

    Background:

    • Traditional interferometers often rely on laser sources, which can have limitations in spectral bandwidth and coherence properties.
    • Achieving high resolution in reflectometry and imaging requires precise control over the light source's characteristics.
    • Measuring group-velocity dispersion is crucial for understanding light-matter interactions and signal propagation.

    Purpose of the Study:

    • To develop a point-fluorescent-source-based white-light interferometer.
    • To demonstrate its utility for high-resolution reflectometry, range-gating imaging, and group-velocity-dispersion measurement.
    • To characterize the performance of the developed fluorescent source for interferometric applications.

    Main Methods:

    • Utilized a laser-pumped point fluorescent source with specific power and spatial coherence.
    • Employed white-light interferometry principles adapted for the fluorescent source.
    • Measured the spectral width and temporal autocorrelation of the fluorescent source.

    Main Results:

    • The point fluorescent source exhibited 9 mW of power and a spatial coherence of 0.97, comparable to a laser beam.
    • The source's 40-nm full width at half maximum (FWHM) spectral width resulted in a temporal autocorrelation width of 19 fs.
    • This temporal width is equivalent to that of 14-fs Gaussian pulses, indicating high temporal resolution.

    Conclusions:

    • The developed point-fluorescent-source-based white-light interferometer is suitable for high-resolution applications.
    • The characteristics of the fluorescent source enable precise measurements in reflectometry, imaging, and dispersion analysis.
    • This approach offers a viable alternative to traditional laser-based interferometers for specific scientific investigations.