Related Experiment Video
Updated: Jun 19, 2026

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography
Published on: January 15, 2014
Structure of the cell wall polysaccharides of probiotic bifidobacteria Bifidobacteriumbifidum BIM B-465
Evelina L Zdorovenko1, Vadim V Kachala, Anastasia V Sidarenka
1N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia. zdorovenkoe@mail.ru
Abstract:
The composition and structure of cell wall polysaccharides of a biotechnologically promising probiotic strain of bifidobacteria Bifidobacteriumbifidum BIM B-465 were established by monosaccharide and methylation analyses along with 2D (1)H-(1)H homonuclear and (1)H-(13)C heteronuclear correlation NMR spectroscopy. The major polysaccharide represents a branched glucogalactan consisting of heptasaccharide repeating units having the following structure: -->6)-alpha-D-Glcp-(1-->3)-beta-D-Galf-(1-->3)-alpha-D-Glcp-(1-->2)-beta-D-Galf-(1-->3)-alpha-D-Galp-(1-->3)-alpha-D-Glcp-(1--> [structure: see the text]. The second isolated polysaccharide is a branched glucan with the main chain of (1-->6)-linked alpha-d-glucopyranose residues, approximately 60% of which are 2-substituted with a single alpha-d-glucopyranosyl group. This polysaccharide is a characteristic of various microorganisms, including some lactobacteria studied earlier.
More Related Videos
Related Concept Videos
Bacterial Cell Wall
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Archaeal Cell Wall
Prokaryotic Cells
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize proteins.
Prokaryotic Cells
Prokaryotic cells

