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[The effect of arsenic sulfide combined with IFN-alpha on K562 cells]
Jing Li1, Shan-Xi Liu, Mei Zhang
1Department of Hematology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. anlj1676@126.com
Aim:
To study if the effect of arsenic sulfide combined with IFN-alpha can be increased on K562 cells.
Methods:
Telomerase activity was determined by PCR-ELISA. Flow cytometry was used to analyze the cell apoptosis. The final concentration of IFN-alpha and arsenic sulfide was 10,000 U/mL and 0.6 mg/L.
Results:
The rates of apoptosis was 37.8% and 37% in K562 cells treated with IFN-alpha or arsenic sulfide alone for 8 days; The rates of apoptosis and inhibition of telomerase activity was 59.9% and 81.2% in K562 cells treated with IFN-alpha and arsenic sulfide simultaneously for 8 days, or 60.37% and 78.8% in K562 cells was treated with arsenic sulfide for 5 days after affected by IFN-alpha for 3 days. 71.3% telomerase activity was inhibited in K562 cells by arsenic sulfide alone for 8 days.
Conclusion:
Combination of arsenic sulfide and IFN-alpha can increase the apoptosis and inhibit the telomerase activity of K562 cells obviously comparing with the two drugs used alone. IFN-alpha maybe promote arsenic sulfide inducing apoptosis of K562 cells.
