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Updated: Jun 19, 2026
09:44
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
Published on: June 13, 2018
Shining new light on 3D cell motility and the metastatic process
Paolo P Provenzano1, Kevin W Eliceiri, Patricia J Keely
1Laboratory for Optical and Computational Instrumentation, and Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA. ppproven@fhcrc.org
Trends in Cell Biology
|October 13, 2009
Summary
Multiphoton microscopy advances optical imaging for studying cancer cell metastasis in 3D environments. This technique helps detect malignant cells and understand cancer cell biology for improved diagnosis and therapy.
Area of Science:
- Biomedical Engineering
- Cancer Biology
- Optical Imaging
Background:
- Studying cancer metastasis requires understanding cell-microenvironment interactions in 3D.
- Mechanistic details of metastasis are challenging to study in 3D environments.
- Optical imaging offers direct observation of single cancer cells during metastasis.
Purpose of the Study:
- To present advances in multiphoton microscopy for cellular imaging.
- To improve the detection of malignant cells.
- To enhance the understanding of cancer cell biology in metastasis.
Main Methods:
- Utilizing multiphoton microscopy for advanced optical imaging.
- Observing single cancer cells in three-dimensional (3D) extracellular matrices.
- Developing novel optical biomarkers linked to cell-motility pathways.
Main Results:
- Demonstrated advances in multiphoton microscopy for cancer research.
- Facilitated direct observation of single cells in metastatic processes.
- Identified optical biomarkers with diagnostic potential for metastasis.
Conclusions:
- Multiphoton microscopy is a promising tool for cancer cell imaging.
- Advances in optical imaging aid in understanding metastasis.
- Optical biomarkers can guide cancer diagnosis and therapy.
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