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Updated: Jun 19, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

High throughput single cell bioinformatics.

Kenneth L Roach1, Kevin R King, Basak E Uygun

  • 1Center for Engineering in Medicine, BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Shriners Hospital for Children, Boston, MA, USA.

Biotechnology Progress
|October 16, 2009
PubMed
Summary
This summary is machine-generated.

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A new microwell array cytometry platform enables single-cell analysis of cell population heterogeneity and stochasticity. This technology reveals distinct cellular responses to oxidative stress, offering detailed insights into biological variability.

Area of Science:

  • Systems biology and bioinformatics
  • Cellular heterogeneity and stochasticity

Background:

  • Bulk measurements can be misleading due to inherent cell population variability.
  • A need exists for techniques combining single-cell resolution, large populations, and time-lapse tracking.

Purpose of the Study:

  • Develop a microwell array cytometry platform for single-cell analysis.
  • Investigate cell heterogeneity and stochasticity in response to oxidative stress.

Main Methods:

  • Fabrication of a microfluidic device with high-density microwell arrays.
  • Automated image processing and data analysis software.
  • Dynamic monitoring of primary hepatocytes using fluorescent probes for mitochondrial membrane potential and free radical generation, followed by bioinformatics clustering (hierarchical, k-means).

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Reusable Single Cell for Iterative Epigenomic Analyses
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Reusable Single Cell for Iterative Epigenomic Analyses

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Last Updated: Jun 19, 2026

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Main Results:

  • Clustering of dynamic data improved comparisons between treatment groups.
  • Distinct cellular response patterns were identified within treatment groups.
  • Heatmaps with hierarchical clustering complemented survival curves in dose-response experiments.

Conclusions:

  • The microwell array cytometry platform is powerful, user-friendly, and provides detailed insights into cell response heterogeneity.
  • This platform has broad applicability for studying cell population heterogeneity, biological stochasticity, and stress responses.