Related Experiment Video
Updated: Jun 19, 2026

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
Published on: October 25, 2018
Spatially resolved DNA brushes on a chip: gene activation by enzymatic cascade
1Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel.
Abstract:
We assemble on a chip spatially resolved DNA polymer brushes with density that can be controlled along continuous gradients, from dilute to dense packing, with 20-30 nm between DNA molecules. Investigation of DNA digestion in a approximately 1 kb DNA brush showed that endonucleases can digest within the brush, yet their activity is impeded at high DNA density and for restriction sites near the bottom. Local gene activation-a form of switch-was then demonstrated by a digestion-ligation cascade between two spatially resolved DNA brushes, leading to the in situ expression of green fluorescent protein upon a diffusion-controlled DNA swapping.
Related Concept Videos
DNA Microarrays
Chromatin Immunoprecipitation- ChIP
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

