Related Experiment Video
Updated: Jun 19, 2026

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers
Published on: August 18, 2017
Figures of merit in the quantification of ibuprofen enantiomers by chiral HPLC
Patrícia Valderrama1, Adriano Lopes Romero, Paulo Mitsuo Imamura
1Department of Analytical Chemistry, Institute of Chemistry, University of Campinas-UNICAMP, P.O. Box 6154, 13083-970, Campinas, SP, Brazil.
Abstract:
The determination of ibuprofen (IBU) enantiomers by chiral high-performance liquid chromatographic is described. The methodology is based on chiral recognition of ibuprofen by a chiral column based on cellulose tris(4-methylbenzoate) coated on silica gel (Chiralcel OJ-H). The mobile phase is n-hexane-2-propanol-trifluoroacetic acid (98:2:0.1, v/v/v). The flow rate was 1.0 mL/min, and UV detection was 254 nm. The samples of ibuprofen were prepared in n-hexane in the concentration range 50-100% of (S)-IBU 1 x 10(-3) mol/L. Calibration and validation method were performed with six and nine samples, respectively. Goodness-of-fit measures represented by correlation coefficient, y-intercept, and slope of the regression line were 0.9836, 21373, 2162, respectively. Average of the relative error of the proposed method was 3.0%, 0.9% (S)-IBU selectivity, and 2162% (S)-IBU-1 sensitivity. The minimum concentration difference between two samples that could be determined in the linear dynamic range was 0.4% (S)-IBU. Limits of detection and quantification were 8.1 and 27.0% (S)-IBU, respectively. These results indicate that the proposed method can be employed for determination of the enantiomeric composition of IBU.
More Related Videos
Related Concept Videos
Properties of Enantiomers and Optical Activity
¹H NMR Chemical Shift Equivalence: Enantiotopic and Diastereotopic Protons
In chiral compounds such as 2-butanol, replacing the methylene hydrogens at C3 produces a pair of...
Racemic Mixtures and the Resolution of Enantiomers
Prochirality
Naming Enantiomers
Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...

