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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 19, 2026

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
07:39

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

Published on: July 21, 2018

Wide-field high-resolution surface-plasmon interference microscopy.

Michael G Somekh1, Graham Stabler, Shugang Liu

  • 1Institute of Biophysics Imaging and Optical Science, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom. mike.somekh@nottingham.ac.uk

Optics Letters
|October 20, 2009
PubMed
Summary
This summary is machine-generated.

A new wide-field interferometric surface-plasmon microscope achieves submicrometer resolution. This advanced optical microscopy technique offers high-resolution imaging with contrast reversals, useful for surface analysis.

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Last Updated: Jun 19, 2026

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Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
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Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Published on: June 5, 2019

Area of Science:

  • Optics and Photonics
  • Surface Science
  • Microscopy

Background:

  • Surface plasmon microscopy is crucial for high-resolution surface imaging.
  • Existing techniques may have limitations in field of view or resolution.
  • Interferometric methods offer phase-sensitive detection.

Purpose of the Study:

  • To introduce a novel wide-field interferometric surface-plasmon microscope.
  • To demonstrate its capability for submicrometer resolution imaging.
  • To analyze contrast mechanisms and instrumental requirements.

Main Methods:

  • Utilizing a speckle-illuminated Linnik interferometer.
  • Implementing a wide-field analog of a scanning heterodyne interferometer.
  • Analyzing contrast reversals at varying defocus levels.

Main Results:

  • Achieved submicrometer lateral resolution.
  • Demonstrated contrast reversals with changes in defocus.
  • Confirmed the system's performance for surface plasmon imaging.

Conclusions:

  • The developed microscope provides high-resolution, wide-field surface plasmon imaging.
  • Understanding contrast mechanisms is key for optimizing imaging.
  • The technique shows promise for detailed surface characterization.