Related Experiment Video
Updated: Jun 19, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
In vitro bioactivity and biocompatibility of dicalcium silicate cements for endodontic use
Chun-Cheng Chen1, Chia-Che Ho, Chan-Hen David Chen
1Department of Dentistry, Chung-Shan Medical University, Taiwan, Republic of China.
Introduction:
The purpose of this study was to examine the in vitro bioactivity and biocompatibility of sol-gel-derived dicalcium silicate cements.
Methods:
The morphology, phase composition, and compressive strength of the novel cement were investigated after immersion in a simulated body fluid for different periods of time. Cement biocompatibility was evaluated by incubating the cement specimens with MG63 human osteoblast-like cells.
Results:
After immersion in a simulated body fluid as little as 1 hour, the cements were covered with clusters of bone-like apatite spherulites. The characteristic peaks of apatite at 2theta = 25.9 degrees and 31.8 - 32.9 degrees appeared. The compressive strength of the cement was increased from the initial strength value of 12.3 MPa to 1-day strength value of 20.2 MPa; these values were significantly different (P < .05). The MG63 cell viability increased 15% and 23% on the cement surfaces when compared with the control on hour 6 and day 7 of incubation, respectively. The cells appeared flat and exhibited intact, well-defined morphology on the cement surface.
Conclusions:
Both bioactivity and biocompatibility of the dicalcium silicate cement consistently make it a potential candidate for endodontic use.
