Related Experiment Video
Updated: Jun 19, 2026
11:28
Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
Published on: March 31, 2012
[Research progress in ARF-GEF gene family]
Shi-Ping Liu1, Lu Wang, Yan-Hong Xue
1State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, China. shipingliu@zju.edu.cn
Yi Chuan = Hereditas
|October 21, 2009
Summary
ADP-ribosylation factor-guanine nucleotide exchange factors (ARF-GEFs) regulate membrane dynamics and protein trafficking. This review summarizes ARF-GEF characteristics, distribution, and their regulatory systems in eukaryotes.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Context:
- ADP-ribosylation factor-guanine nucleotide exchange factors (ARF-GEFs) are crucial regulators of membrane trafficking.
- These large ARF-GEFs are highly conserved across eukaryotic organisms.
- Recent research has focused on understanding ARF-GEF structure, localization, and function.
Purpose:
- To review the characteristics and species distribution of ARF-GEFs.
- To summarize recent advancements in ARF-GEF research.
- To outline the regulatory systems governing ARF-GEF activity.
Summary:
- ARF-GEFs catalyze GDP/GTP exchange on ADP-ribosylation factors, impacting essential cellular processes.
- The review covers conserved features and diverse distribution of ARF-GEFs in various species.
- Key findings on ARF-GEF regulation, structure, and physiological roles are presented.
Impact:
- Provides a comprehensive overview of ARF-GEFs for researchers in cell biology and related fields.
- Highlights the importance of ARF-GEFs in fundamental cellular mechanisms like membrane dynamics.
- Facilitates further investigation into ARF-GEF-mediated pathways and potential therapeutic targets.
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