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Updated: May 27, 2026
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Synchronous fluorescence as a rapid method for the simultaneous determination of folic acid and riboflavin in
Yang Wang1, Pei-Hua Zhu, Tian Tian
1College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China. wangyangyz@yahoo.cn
Abstract:
A rapid synchronous spectrofluorimetric method was first developed for the simultaneous determination of folic acid and riboflavin in nutrimental beverages. Folic acid could be detected by using H(2)O(2) plus Cu(II) as oxidation system to produce pterine-6-carboxylic acid, which had strong fluorescence in aqueous solution, and riboflavin itself was obviously fluorescent. Various operational parameters were thoroughly discussed in terms of their effects on the fluorescence signals, including instrumental parameters, concentration of the oxidation system, and pH. Under optimum conditions, the calibration curves were linear in the ranges of 100-250 μg/L for folic acid and 1-250 μg/L for riboflavin, and the detection limits were 2.0 and 0.014 μg/L, respectively. In addition, this method was applied to the determination of folic acid and riboflavin in nutrimental beverages with satisfactory results.
