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Related Experiment Video

Updated: Jun 19, 2026

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
07:54

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

Proteomic analysis in NSAIDs-treated primary cardiomyocytes.

Seon Mi Baek1, Jin Sook Ahn, Hae Sook Noh

  • 1Department of Biochemistry, Institute of Health Sciences, Gyeongsang National University School of Medicine, JinJu, Republic of Korea.

Journal of Proteomics
|October 24, 2009
PubMed
Summary

Non-steroidal anti-inflammatory drugs (NSAIDs) can cause cardiovascular toxicity. This study identified Septin-8 as a protein whose expression level varies with NSAID toxicity in cardiomyocytes.

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Area of Science:

  • Cardiovascular pharmacology
  • Proteomics
  • Cellular toxicology

Background:

  • Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used but some have been withdrawn due to cardiovascular toxicity.
  • Understanding the molecular mechanisms of NSAID-induced cardiotoxicity is crucial for drug safety.

Purpose of the Study:

  • To identify proteins that respond to cellular toxicity induced by various NSAIDs in cardiomyocytes.
  • To investigate the differential protein expression patterns related to varying cardiovascular risks of NSAIDs.

Main Methods:

  • Primary culture of cardiomyocytes treated with seven different NSAIDs (celecoxib, rofecoxib, valdecoxib, diclofenac, naproxen, ibuprofen, meloxicam).
  • Two-dimensional (2-D) proteomic analysis to compare protein expression profiles.

Related Experiment Videos

Last Updated: Jun 19, 2026

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
07:54

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

  • Quantification and identification of differentially expressed proteins.

    • Main Results:

    • NSAID treatment altered protein expression, with higher decreases in proteins observed with more cardiotoxic NSAIDs (celecoxib, rofecoxib).
    • Identified proteins were involved in cellular stress, muscle contraction, and membrane organization.
    • Septin-8 expression was inversely correlated with NSAID cardiotoxicity, showing lower levels with highly toxic drugs and stable levels with less toxic ones.

    Conclusions:

    • Septin-8 exhibits a differential response to NSAIDs based on their cardiotoxic potential.
    • This finding suggests Septin-8 may serve as a biomarker for NSAID-induced cardiotoxicity.
    • Further research into Septin-8's role could inform the development of safer NSAIDs.