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Studies on the synthesis of basic proteins and antigenic components in measles virus infected cells
I Polna1, F Rzendowska, J Beker
1Department of Virology, Department of Radiation Protection and Radiobiology, National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland.
Abstract:
The synthesis of histone f1 fraction in synchronous culture of (3H)-L-lysine labeled HeLa cells was inhibited 30 to 45 hours earlier and to a higher degree after infection with attenuated L-16 strain of the measles virus than after infection with laboratory Edmonston strain. Dithiothreitol and triton X-100 were used to isolate a cytoplasmatic component (K-2c) lacking infectivity but associated with hemagglutinating activity, from both strains. The component from both strain induced the synthesis of measles antibodies detected by the hemagglutination inhibition, neutralization and immunodiffusion techniques. The antibody titers in the sera of animals vaccinated with K-2c of L-16 strain were similar or higher than for K-2c of the Edmonston strain.
Insights
Measles virus infection, particularly the L-16 strain, significantly inhibits histone synthesis in HeLa cells. A non-infectious cytoplasmic component (K-2c) from both strains effectively induces measles antibodies.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Measles virus infection impacts host cell processes, including histone synthesis.
- Different measles virus strains may exhibit varying effects on cellular functions.
- Characterizing viral components is crucial for understanding pathogenesis and vaccine development.
Purpose of the Study:
- To investigate the differential effects of measles virus strains (L-16 and Edmonston) on histone F1 synthesis in HeLa cells.
- To isolate and characterize a cytoplasmic component (K-2c) associated with hemagglutinating activity.
- To evaluate the immunogenicity of the K-2c component in inducing measles antibodies.
Main Methods:
- Synchronous HeLa cell cultures labeled with (3H)-L-lysine.
- Isolation of a cytoplasmic component (K-2c) using dithiothreitol and Triton X-100.
- Detection of measles antibodies via hemagglutination inhibition, neutralization, and immunodiffusion assays.
- Vaccination of animals with K-2c components and subsequent antibody titer analysis.
Main Results:
- Infection with the attenuated L-16 measles virus strain caused earlier and greater inhibition of histone F1 synthesis compared to the Edmonston strain.
- A non-infectious cytoplasmic component (K-2c) with hemagglutinating activity was isolated from both viral strains.
- The K-2c component from both strains successfully induced measles antibodies in vitro and in vivo.
- Antibody titers in animals vaccinated with L-16 K-2c were comparable or higher than those vaccinated with Edmonston K-2c.
Conclusions:
- The L-16 measles virus strain exhibits a more pronounced inhibitory effect on histone synthesis than the Edmonston strain.
- The K-2c component represents a potentially valuable antigen for measles vaccine development due to its ability to elicit a robust antibody response.
- Further research into the mechanisms of histone synthesis inhibition and the immunomodulatory properties of K-2c is warranted.
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