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Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients
Published on: November 19, 2019
TRPV6 alleles do not influence prostate cancer progression
Thorsten Kessler1, Ulrich Wissenbach, Rainer Grobholz
1Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg/Saar, Germany. kesslerthorsten@googlemail.com
BMC Cancer
|October 28, 2009
Summary
The transient receptor potential, subfamily V, member 6 (TRPV6) genotype does not correlate with prostate cancer onset, Gleason score, or tumor stage, despite its altered expression in aggressive forms of the disease.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Transient receptor potential, subfamily V, member 6 (TRPV6) is a Ca(2+) selective cation channel.
- TRPV6 transcripts are found in advanced prostate cancer but not in healthy tissue.
- Two allelic variants, TRPV6a and TRPV6b, exist for the human TRPV6 gene.
Purpose of the Study:
- To investigate the correlation between the trpv6a allele and prostate cancer onset.
- To determine the association of the trpv6a allele with Gleason score and tumor stage.
Main Methods:
- Genotyping of TRPV6 gene single nucleotide polymorphisms (SNPs) using restriction fragment length polymorphism or sequencing.
- RT-PCR to analyze RNA isolated from prostate tissue.
- Chi-Square test for data analysis.
Main Results:
- The TRPV6b genotype predominated (86%) in healthy individuals; no homozygous TRPV6a carriers were found.
- TRPV6b allele frequency was 87% in prostate adenocarcinoma samples, with no correlation to Gleason score or tumor stage.
- Allele frequencies in healthy individuals and prostate cancer patients were not significantly different.
Conclusions:
- TRPV6 allele frequencies do not differ significantly between healthy individuals and prostate cancer patients.
- TRPV6 genotype is not correlated with prostate cancer onset, Gleason score, or tumor stage.
- Despite altered expression correlating with aggressive prostate cancer, the TRPV6 genotype lacks predictive value for disease characteristics.
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